SibEnzyme REBase

restriction enzymes database

Name search

Pvu II

Enzyme name Pvu II
Prototype PvuII
SKU SE-E111
Turbo version Available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CAG▼CTG …3'
3'… GTC▲GAC …5'
Source An E.coli strain that carries the cloned Pvu II gene from Proteus vulgaris
Optimal buffer SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
25100252525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme approximately 70% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer G, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  2. S.Kh. Degtyarev, V.E. Repin, N.I. Rechkunova Proteus vulgaris - a strain producer of restriction endonuclease PvuII. // Soviet Union patent SU 1632974 (1991). (In Russian)

Sequence search

Restriction enzyme search by DNA sequence
Only A, G, C, T are allowed. Maximum length is 50 nucleotides.

Enzyme list

Pvu II

Enzyme name Pvu II
Prototype PvuII
SKU SE-E111
Turbo version Available
High-concentration version Not available
Restriction type Type IIPiType IIP enzymes are classical Type II restriction enzymes with palindromic recognition sequences and symmetric cleavage sites.
Recognition site
5'… CAG▼CTG …3'
3'… GTC▲GAC …5'
Source An E.coli strain that carries the cloned Pvu II gene from Proteus vulgaris
Optimal buffer SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
25100252525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme approximately 70% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer G, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  2. S.Kh. Degtyarev, V.E. Repin, N.I. Rechkunova Proteus vulgaris - a strain producer of restriction endonuclease PvuII. // Soviet Union patent SU 1632974 (1991). (In Russian)

Isoshizomers

Isoschizomers and neoschizomers

SE enzymeRecognition siteKnown analogsTypeRecognition site
Aat II
5'… GACGT▼C …3'
3'… C▲TGCAG …5'
Zra I
Neoschizomer
5'… GAC▼GTC …3'
3'… CTG▲CAG …5'
Acc16 I
5'… TGC▼GCA …3'
3'… ACG▲CGT …5'
AviII
FspI
MstI
NsbI
Isoschizomer
5'… TGC▼GCA …3'
3'… ACG▲CGT …5'
Acc36 I
5'… ACCTGC(N)4▼ …3'
3'… TGGACG(N)8▲ …5'
BfuAI
BspMI
BveI
Isoschizomer
5'… ACCTGC(N)4▼ …3'
3'… TGGACG(N)8▲ …5'
Acc65 I
5'… G▼GTACC …3'
3'… CCATG▲G …5'
Asp718I
Isoschizomer
5'… G▼GTACC …3'
3'… CCATG▲G …5'
Kpn I
Neoschizomer
5'… GGTAC▼C …3'
3'… C▲CATGG …5'
AccB1 I
5'… G▼GYRCC …3'
3'… CCRYG▲G …5'
BanI
BshNI
BspT107I
HgiCI
Isoschizomer
5'… G▼GYRCC …3'
3'… CCRYG▲G …5'
AccB7 I
5'… CCANNNN▼NTGG …3'
3'… GGTN▲NNNNACC …5'
PflBI
PflMI
Van91I
Isoschizomer
5'… CCANNNN▼NTGG …3'
3'… GGTN▲NNNNACC …5'
AccBS I
5'… GAG▼CGG …3'
3'… CTC▲GCC …5'
BsrBI
MbiI
Isoschizomer
5'… GAG▼CGG …3'
3'… CTC▲GCC …5'
Acl I
5'… AA▼CGTT …3'
3'… TTGC▲AA …5'
Psp1406I
Isoschizomer
5'… AA▼CGTT …3'
3'… TTGC▲AA …5'
AclW I
5'… GGATC(N)4▼ …3'
3'… CCTAG(N)5▲ …5'
AlwI
BinI
BspPI
Isoschizomer
5'… GGATC(N)4▼ …3'
3'… CCTAG(N)5▲ …5'
Aco I
5'… Y▼GGCCR …3'
3'… RCCGG▲Y …5'
CfrI
EaeI
Isoschizomer
5'… Y▼GGCCR …3'
3'… RCCGG▲Y …5'
Acs I
5'… R▼AATTY …3'
3'… YTTAA▲R …5'
ApoI
XapI
Isoschizomer
5'… R▼AATTY …3'
3'… YTTAA▲R …5'
Acu I
5'… CTGAAG(N)16▼ …3'
3'… GACTTC(N)14▲ …5'
Eco57I
Isoschizomer
5'… CTGAAG(N)16▼ …3'
3'… GACTTC(N)14▲ …5'
Afe I
5'… AGC▼GCT …3'
3'… TCG▲CGA …5'
Aor51HI
Eco47III
FunI
Isoschizomer
5'… AGC▼GCT …3'
3'… TCG▲CGA …5'
Ahl I
5'… A▼CTAGT …3'
3'… TGATC▲A …5'
BcuI
SpeI
Isoschizomer
5'… A▼CTAGT …3'
3'… TGATC▲A …5'
Ajn I
Psp6 I
5'… ▼CCWGG …3'
3'… GGWCC▲ …5'
EcoRII
PspGI
Isoschizomer
5'… ▼CCWGG …3'
3'… GGWCC▲ …5'
BptI
BseBI
Bst2U I
BstNI
BstOI
MvaI
Neoschizomer
5'… CC▼WGG …3'
3'… GGW▲CC …5'
Ama87 I
5'… C▼YCGRG …3'
3'… GRGCY▲C …5'
AvaI
BsiHKCI
BsoBI
Eco88I
NspIII
Isoschizomer
5'… C▼YCGRG …3'
3'… GRGCY▲C …5'
Apa I
5'… GGGCC▼C …3'
3'… C▲CCGGG …5'
Bsp120I
PspOM I
Neoschizomer
5'… G▼GGCCC …3'
3'… CCCGG▲G …5'
AsiG I
5'… A▼CCGGT …3'
3'… TGGCC▲A …5'
AgeI
BshTI
CspAI
PinAI
Isoschizomer
5'… A▼CCGGT …3'
3'… TGGCC▲A …5'
AsiS I
Rga I
5'… GCGAT▼CGC …3'
3'… CGC▲TAGCG …5'
SgfI
Isoschizomer
5'… GCGAT▼CGC …3'
3'… CGC▲TAGCG …5'
AspA2 I
5'… C▼CTAGG …3'
3'… GGATC▲C …5'
AvrII
BlnI
XmaJI
Isoschizomer
5'… C▼CTAGG …3'
3'… GGATC▲C …5'
AspLE I
BstHH I
5'… GCG▼C …3'
3'… C▲GCG …5'
CfoI
HhaI
Isoschizomer
5'… GCG▼C …3'
3'… C▲GCG …5'
Hin6I
HinP1I
HspA I
Neoschizomer
5'… G▼CGC …3'
3'… CGC▲G …5'
AspS9 I
5'… G▼GNCC …3'
3'… CCNG▲G …5'
AsuI
Cfr13I
PspPI
Sau96I
Isoschizomer
5'… G▼GNCC …3'
3'… CCNG▲G …5'
AsuC2 I
5'… CC▼SGG …3'
3'… GGS▲CC …5'
BcnI
CauII
NciI
Isoschizomer
5'… CC▼SGG …3'
3'… GGS▲CC …5'
AsuHP I
5'… GGTGA(N)8▼ …3'
3'… CCACT(N)7▲ …5'
HphI
Isoschizomer
5'… GGTGA(N)8▼ …3'
3'… CCACT(N)7▲ …5'
AsuNH I
5'… G▼CTAGC …3'
3'… CGATC▲G …5'
NheI
Isoschizomer
5'… G▼CTAGC …3'
3'… CGATC▲G …5'
Bmt I
Neoschizomer
5'… GCTAG▼C …3'
3'… C▲GATCG …5'
Bbv12 I
5'… GWGCW▼C …3'
3'… C▲WCGWG …5'
Alw21I
BsiHKAI
HgiAI
Isoschizomer
5'… GWGCW▼C …3'
3'… C▲WCGWG …5'
Bme18 I
5'… G▼GWCC …3'
3'… CCWG▲G …5'
AvaII
Eco47I
SinI
VpaK11BI
Isoschizomer
5'… G▼GWCC …3'
3'… CCWG▲G …5'
Bmu I
5'… ACTGGG(N)5▼ …3'
3'… TGACCC(N)4▲ …5'
BfiI
BmrI
Isoschizomer
5'… ACTGGG(N)5▼ …3'
3'… TGACCC(N)4▲ …5'
Bpm I
5'… CTGGAG(N)16▼ …3'
3'… GACCTC(N)14▲ …5'
GsuI
Isoschizomer
5'… CTGGAG(N)16▼ …3'
3'… GACCTC(N)14▲ …5'
Bpu14 I
5'… TT▼CGAA …3'
3'… AAGC▲TT …5'
AsuII
Bsp119I
BspT104I
BstBI
Csp45I
NspV
SfuI
Isoschizomer
5'… TT▼CGAA …3'
3'… AAGC▲TT …5'
Bsa29 I
5'… AT▼CGAT …3'
3'… TAGC▲TA …5'
BanIII
BseCI
BspDI
BspXI
Bsu15I
BsuTUI
ClaI
ZhoI
Isoschizomer
5'… AT▼CGAT …3'
3'… TAGC▲TA …5'
Bsc4 I
5'… CCNNNNN▼NNGG …3'
3'… GGNN▲NNNNNCC …5'
BseLI
BsiYI
BslI
Isoschizomer
5'… CCNNNNN▼NNGG …3'
3'… GGNN▲NNNNNCC …5'
Bse1 I
5'… ACTGGN▼ …3'
3'… TGAC▲CN …5'
BseNI
BsrI
BsrSI
Isoschizomer
5'… ACTGGN▼ …3'
3'… TGAC▲CN …5'
Bse3D I
5'… GCAATGNN▼ …3'
3'… CGTTAC▲NN …5'
BseMI
BsrDI
Isoschizomer
5'… GCAATGNN▼ …3'
3'… CGTTAC▲NN …5'
Bse8 I
5'… GATNN▼NNATC …3'
3'… CTANN▲NNTAG …5'
BsaBI
BseJI
MamI
Isoschizomer
5'… GATNN▼NNATC …3'
3'… CTANN▲NNTAG …5'
Bse21 I
5'… CC▼TNAGG …3'
3'… GGANT▲CC …5'
AxyI
Bsu36I
Eco81I
SauI
Isoschizomer
5'… CC▼TNAGG …3'
3'… GGANT▲CC …5'
Bse118 I
5'… R▼CCGGY …3'
3'… YGGCC▲R …5'
BsrFI
BssAI
Cfr10I
Isoschizomer
5'… R▼CCGGY …3'
3'… YGGCC▲R …5'
BseP I
5'… G▼CGCGC …3'
3'… CGCGC▲G …5'
BssHII
PauI
Isoschizomer
5'… G▼CGCGC …3'
3'… CGCGC▲G …5'
BseX3 I
5'… C▼GGCCG …3'
3'… GCCGG▲C …5'
BstZI
EagI
EclXI
Eco52I
XmaIII
Isoschizomer
5'… C▼GGCCG …3'
3'… GCCGG▲C …5'
BslF I
5'… GGGAC(N)10▼ …3'
3'… CCCTG(N)14▲ …5'
BsmFI
FaqI
Isoschizomer
5'… GGGAC(N)10▼ …3'
3'… CCCTG(N)14▲ …5'
Bso31 I
5'… GGTCTCN▼ …3'
3'… CCAGAG(N)5▲ …5'
BsaI
BspTNI
Eco31I
Isoschizomer
5'… GGTCTCN▼ …3'
3'… CCAGAG(N)5▲ …5'
Bsp13 I
5'… T▼CCGGA …3'
3'… AGGCC▲T …5'
AccIII
Aor13HI
BlfI
BseAI
BspEI
BspMII
Kpn2I
MroI
Isoschizomer
5'… T▼CCGGA …3'
3'… AGGCC▲T …5'
Bsp19 I
5'… C▼CATGG …3'
3'… GGTAC▲C …5'
NcoI
Isoschizomer
5'… C▼CATGG …3'
3'… GGTAC▲C …5'
Bsp1720 I
5'… GC▼TNAGC …3'
3'… CGANT▲CG …5'
BlpI
Bpu1102I
CelII
EspI
Isoschizomer
5'… GC▼TNAGC …3'
3'… CGANT▲CG …5'
BspAC I
5'… C▼CGC …3'
3'… GGC▲G …5'
AciI
SsiI
Isoschizomer
5'… C▼CGC …3'
3'… GGC▲G …5'
BssEC I
5'… C▼CNNGG …3'
3'… GGNNC▲C …5'
BsaJI
BseDI
SecI
Isoschizomer
5'… C▼CNNGG …3'
3'… GGNNC▲C …5'
BssNA I
5'… GTA▼TAC …3'
3'… CAT▲ATG …5'
Bst1107I
BstZ17I
Isoschizomer
5'… GTA▼TAC …3'
3'… CAT▲ATG …5'
BssT1 I
Erh I
5'… C▼CWWGG …3'
3'… GGWWC▲C …5'
Eco130I
EcoT14I
StyI
Isoschizomer
5'… C▼CWWGG …3'
3'… GGWWC▲C …5'
Bst2B I
5'… C▼TCGTG …3'
3'… GAGCA▲C …5'
BauI
BsiI
BssSI
Isoschizomer
5'… C▼TCGTG …3'
3'… GAGCA▲C …5'
Bst4C I
5'… ACN▼GT …3'
3'… TG▲NCA …5'
HpyCH4III
TaaI
Tsp4CI
Isoschizomer
5'… ACN▼GT …3'
3'… TG▲NCA …5'
Bst6 I
5'… CTCTTCN▼ …3'
3'… GAGAAG(N)4▲ …5'
Eam1104I
EarI
Ksp632I
Isoschizomer
5'… CTCTTCN▼ …3'
3'… GAGAAG(N)4▲ …5'
BstAC I
5'… GR▼CGYC …3'
3'… CYGC▲RG …5'
AcyI
BsaHI
Hin1I
Hsp92I
Isoschizomer
5'… GR▼CGYC …3'
3'… CYGC▲RG …5'
BstAF I
5'… C▼TTAAG …3'
3'… GAATT▲C …5'
AflII
BfrI
BspTI
Bst98I
MspCI
Isoschizomer
5'… C▼TTAAG …3'
3'… GAATT▲C …5'
BstAP I
5'… GCANNNN▼NTGC …3'
3'… CGTN▲NNNNACG …5'
ApaBI
Neoschizomer
5'… GCANNNNN▼TGC …3'
3'… CGT▲NNNNNACG …5'
BstAU I
5'… T▼GTACA …3'
3'… ACATG▲T …5'
Bsp1407I
BsrGI
SspBI
Isoschizomer
5'… T▼GTACA …3'
3'… ACATG▲T …5'
BstBA I
5'… YAC▼GTR …3'
3'… RTG▲CAY …5'
BsaAI
Isoschizomer
5'… YAC▼GTR …3'
3'… RTG▲CAY …5'
BstC8 I
5'… GCN▼NGC …3'
3'… CGN▲NCG …5'
Cac8I
Isoschizomer
5'… GCN▼NGC …3'
3'… CGN▲NCG …5'
BstDE I
5'… C▼TNAG …3'
3'… GANT▲C …5'
DdeI
HpyF3I
Isoschizomer
5'… C▼TNAG …3'
3'… GANT▲C …5'
BstDS I
5'… C▼CRYGG …3'
3'… GGYRC▲C …5'
BtgI
DsaI
Isoschizomer
5'… C▼CRYGG …3'
3'… GGYRC▲C …5'
BstEN I
5'… CCTNN▼NNNAGG …3'
3'… GGANNN▲NNTCC …5'
EcoNI
XagI
Isoschizomer
5'… CCTNN▼NNNAGG …3'
3'… GGANNN▲NNTCC …5'
BstF5 I
5'… GGATGNN▼ …3'
3'… CCTAC▲NN …5'
BseGI
Isoschizomer
5'… GGATGNN▼ …3'
3'… CCTAC▲NN …5'
Fok I
Neoschizomer
5'… GGATG(N)9▼ …3'
3'… CCTAC(N)13▲ …5'
BstFN I
5'… CG▼CG …3'
3'… GC▲GC …5'
AccII
Bsh1236I
BstUI
FnuDII
MvnI
Isoschizomer
5'… CG▼CG …3'
3'… GC▲GC …5'
BstH2 I
5'… RGCGC▼Y …3'
3'… Y▲CGCGR …5'
Bsp143II
HaeII
Isoschizomer
5'… RGCGC▼Y …3'
3'… Y▲CGCGR …5'
BstKT I
5'… GAT▼C …3'
3'… C▲TAG …5'
BfuCI
Bsp143I
BstMB I
DpnII
Kzo9 I
MboI
NdeII
Sau3AI
Neoschizomer
5'… ▼GATC …3'
3'… CTAG▲ …5'
Mal I
5'… G(mA)▼TC …3'
3'… CT▲(mA)G …5'
DpnI
Isoschizomer
5'… G(mA)▼TC …3'
3'… CT▲(mA)G …5'
BstMA I
5'… GTCTCN▼ …3'
3'… CAGAG(N)5▲ …5'
Alw26I
BsmAI
Isoschizomer
5'… GTCTCN▼ …3'
3'… CAGAG(N)5▲ …5'
BstMC I
5'… CGRY▼CG …3'
3'… GC▲YRGC …5'
Bsh1285I
BsiEI
McrI
Isoschizomer
5'… CGRY▼CG …3'
3'… GC▲YRGC …5'
BstMW I
5'… GCNNNNN▼NNGC …3'
3'… CGNN▲NNNNNCG …5'
HpyF10VI
MwoI
Isoschizomer
5'… GCNNNNN▼NNGC …3'
3'… CGNN▲NNNNNCG …5'
BstNS I
5'… RCATG▼Y …3'
3'… Y▲GTACR …5'
NspI
XceI
Isoschizomer
5'… RCATG▼Y …3'
3'… Y▲GTACR …5'
BstPA I
5'… GACNN▼NNGTC …3'
3'… CTGNN▲NNCAG …5'
BoxI
PshAI
Isoschizomer
5'… GACNN▼NNGTC …3'
3'… CTGNN▲NNCAG …5'
BstSC I
5'… ▼CCNGG …3'
3'… GGNCC▲ …5'
BssKI
StyD4I
Isoschizomer
5'… ▼CCNGG …3'
3'… GGNCC▲ …5'
Bme1390I
MspR9 I
ScrFI
Neoschizomer
5'… CC▼NGG …3'
3'… GGN▲CC …5'
BstSF I
5'… C▼TRYAG …3'
3'… GAYRT▲C …5'
BfmI
BpcI
SfcI
SfeI
Isoschizomer
5'… C▼TRYAG …3'
3'… GAYRT▲C …5'
BstSN I
5'… TAC▼GTA …3'
3'… ATG▲CAT …5'
Eco105I
SnaBI
Isoschizomer
5'… TAC▼GTA …3'
3'… ATG▲CAT …5'
BstV1 I
5'… GCAGC(N)8▼ …3'
3'… CGTCG(N)12▲ …5'
BbvI
BseXI
Isoschizomer
5'… GCAGC(N)8▼ …3'
3'… CGTCG(N)12▲ …5'
BstV2 I
5'… GAAGAC(N)2▼ …3'
3'… CTTCTG(N)6▲ …5'
BbsI
BbvII
BpiI
BpuAI
Isoschizomer
5'… GAAGAC(N)2▼ …3'
3'… CTTCTG(N)6▲ …5'
BstX2 I
5'… R▼GATCY …3'
3'… YCTAG▲R …5'
BstYI
MflI
PsuI
XhoII
Isoschizomer
5'… R▼GATCY …3'
3'… YCTAG▲R …5'
Bsu I
5'… GTATCC(N)6▼ …3'
3'… CATAGG(N)5▲ …5'
BciVI
Isoschizomer
5'… GTATCC(N)6▼ …3'
3'… CATAGG(N)5▲ …5'
BsuR I
Hae III
5'… GG▼CC …3'
3'… CC▲GG …5'
BshFI
BspANI
PhoI
Isoschizomer
5'… GG▼CC …3'
3'… CC▲GG …5'
Btr I
5'… CAC▼GTC …3'
3'… GTG▲CAG …5'
BmgBI
Isoschizomer
5'… CAC▼GTC …3'
3'… GTG▲CAG …5'
CciN I
5'… GC▼GGCCGC …3'
3'… CGCCGG▲CG …5'
NotI
Isoschizomer
5'… GC▼GGCCGC …3'
3'… CGCCGG▲CG …5'
Dra I
5'… TTT▼AAA …3'
3'… AAA▲TTT …5'
AhaIII
Isoschizomer
5'… TTT▼AAA …3'
3'… AAA▲TTT …5'
Dra III
5'… CACNNN▼GTG …3'
3'… GTG▲NNNCAC …5'
AdeI
Isoschizomer
5'… CACNNN▼GTG …3'
3'… GTG▲NNNCAC …5'
Dri I
5'… GACNNN▼NNGTC …3'
3'… CTGNN▲NNNCAG …5'
AhdI
AspEI
Eam1105I
EclHKI
Isoschizomer
5'… GACNNN▼NNGTC …3'
3'… CTGNN▲NNNCAG …5'
DseD I
5'… GACNNNN▼NNGTC …3'
3'… CTGNN▲NNNNCAG …5'
AasI
DrdI
Isoschizomer
5'… GACNNNN▼NNGTC …3'
3'… CTGNN▲NNNNCAG …5'
EcoICR I
5'… GAG▼CTC …3'
3'… CTC▲GAG …5'
Ecl136II
Isoschizomer
5'… GAG▼CTC …3'
3'… CTC▲GAG …5'
Psp124B I
SacI
SstI
Neoschizomer
5'… GAGCT▼C …3'
3'… C▲TCGAG …5'
EcoR I
5'… G▼AATTC …3'
3'… CTTAA▲G …5'
FunII
Isoschizomer
5'… G▼AATTC …3'
3'… CTTAA▲G …5'
EcoR V
5'… GAT▼ATC …3'
3'… CTA▲TAG …5'
Eco32I
Isoschizomer
5'… GAT▼ATC …3'
3'… CTA▲TAG …5'
Ege I
5'… GGC▼GCC …3'
3'… CCG▲CGG …5'
EheI
SfoI
Isoschizomer
5'… GGC▼GCC …3'
3'… CCG▲CGG …5'
BbeI
Neoschizomer
5'… GGCGC▼C …3'
3'… C▲CGCGG …5'
KasI
Neoschizomer
5'… G▼GCGCC …3'
3'… CCGCG▲G …5'
Mly113 I
NarI
Neoschizomer
5'… GG▼CGCC …3'
3'… CCGC▲GG …5'
Fae I
5'… CATG▼ …3'
3'… ▲GTAC …5'
Hin1II
Hsp92II
NlaIII
Isoschizomer
5'… CATG▼ …3'
3'… ▲GTAC …5'
CviAII
Neoschizomer
5'… C▼ATG …3'
3'… GTA▲C …5'
Fat I
Neoschizomer
5'… ▼CATG …3'
3'… GTAC▲ …5'
Fau I
5'… CCCGC(N)4▼ …3'
3'… GGGCG(N)6▲ …5'
SmuI
Isoschizomer
5'… CCCGC(N)4▼ …3'
3'… GGGCG(N)6▲ …5'
FauND I
5'… CA▼TATG …3'
3'… GTAT▲AC …5'
NdeI
Isoschizomer
5'… CA▼TATG …3'
3'… GTAT▲AC …5'
Fbl I
5'… GT▼mKAC …3'
3'… CAKm▲TG …5'
AccI
XmiI
Isoschizomer
5'… GT▼mKAC …3'
3'… CAKm▲TG …5'
BstF5 I
5'… GGATGNN▼ …3'
3'… CCTAC▲NN …5'
Fok I
Neoschizomer
5'… GGATG(N)9▼ …3'
3'… CCTAC(N)13▲ …5'
BseGI
Neoschizomer
5'… GGATGNN▼ …3'
3'… CCTAC▲NN …5'
FriO I
5'… GRGCY▼C …3'
3'… C▲YCGRG …5'
BanII
Eco24I
EcoT38I
HgiJII
Isoschizomer
5'… GRGCY▼C …3'
3'… C▲YCGRG …5'
Fsp4H I
5'… GC▼NGC …3'
3'… CGN▲CG …5'
Fnu4HI
ItaI
SatI
Isoschizomer
5'… GC▼NGC …3'
3'… CGN▲CG …5'
Hga I
5'… GACGC(N)5▼ …3'
3'… CTGCG(N)10▲ …5'
CseI
Isoschizomer
5'… GACGC(N)5▼ …3'
3'… CTGCG(N)10▲ …5'
Hind II
5'… GTY▼RAC …3'
3'… CAR▲YTG …5'
HincII
Isoschizomer
5'… GTY▼RAC …3'
3'… CAR▲YTG …5'
Hpa I
5'… GTT▼AAC …3'
3'… CAA▲TTG …5'
KspAI
Isoschizomer
5'… GTT▼AAC …3'
3'… CAA▲TTG …5'
Hpa II
Msp I
5'… C▼CGG …3'
3'… GGC▲C …5'
BsiSI
HapII
Isoschizomer
5'… C▼CGG …3'
3'… GGC▲C …5'
HpySE526 I
5'… A▼CGT …3'
3'… TGC▲A …5'
MaeII
Isoschizomer
5'… A▼CGT …3'
3'… TGC▲A …5'
Ksp22 I
5'… T▼GATCA …3'
3'… ACTAG▲T …5'
BclI
FbaI
Isoschizomer
5'… T▼GATCA …3'
3'… ACTAG▲T …5'
Mab I
5'… A▼CCWGGT …3'
3'… TGGWCC▲A …5'
SexAI
Isoschizomer
5'… A▼CCWGGT …3'
3'… TGGWCC▲A …5'
Mfe I
5'… C▼AATTG …3'
3'… GTTAA▲C …5'
MunI
Isoschizomer
5'… C▼AATTG …3'
3'… GTTAA▲C …5'
Mhl I
5'… GDGCH▼C …3'
3'… C▲HCGDG …5'
Bsp1286I
SduI
Isoschizomer
5'… GDGCH▼C …3'
3'… C▲HCGDG …5'
Mox20 I
5'… TGG▼CCA …3'
3'… ACC▲GGT …5'
BalI
MlsI
MluNI
MscI
Msp20I
Isoschizomer
5'… TGG▼CCA …3'
3'… ACC▲GGT …5'
MroN I
5'… G▼CCGGC …3'
3'… CGGCC▲G …5'
NgoMIV
Isoschizomer
5'… G▼CCGGC …3'
3'… CGGCC▲G …5'
NaeI
Neoschizomer
5'… GCC▼GGC …3'
3'… CGG▲CCG …5'
PdiI
Neoschizomer
5'… GCC▼GGC …3'
3'… CGG▲CCG …5'
MroX I
5'… GAANN▼NNTTC …3'
3'… CTTNN▲NNAAG …5'
Asp700I
PdmI
XmnI
Isoschizomer
5'… GAANN▼NNTTC …3'
3'… CTTNN▲NNAAG …5'
MspA1 I
5'… CmG▼CKG …3'
3'… GKC▲GmC …5'
NspBII
Isoschizomer
5'… CmG▼CKG …3'
3'… GKC▲GmC …5'
Nru I
5'… TCG▼CGA …3'
3'… AGC▲GCT …5'
Bsp68I
Isoschizomer
5'… TCG▼CGA …3'
3'… AGC▲GCT …5'
PalA I
5'… GG▼CGCGCC …3'
3'… CCGCGC▲GG …5'
AscI
AsoI
SgsI
Isoschizomer
5'… GG▼CGCGCC …3'
3'… CCGCGC▲GG …5'
Pce I
5'… AGG▼CCT …3'
3'… TCC▲GGA …5'
AatI
Eco147I
SseBI
StuI
Isoschizomer
5'… AGG▼CCT …3'
3'… TCC▲GGA …5'
Pci I
5'… A▼CATGT …3'
3'… TGTAC▲A …5'
BspLU11I
PscI
Isoschizomer
5'… A▼CATGT …3'
3'… TGTAC▲A …5'
PciS I
5'… GCTCTTCN▼ …3'
3'… CGAGAAG(N)4▲ …5'
LguI
SapI
Isoschizomer
5'… GCTCTTCN▼ …3'
3'… CGAGAAG(N)4▲ …5'
Pct I
5'… GAATGCN▼ …3'
3'… CTTAC▲GN …5'
BsaMI
BsmI
Mva1269I
Isoschizomer
5'… GAATGCN▼ …3'
3'… CTTAC▲GN …5'
Ple19 I
5'… CGAT▼CG …3'
3'… GC▲TAGC …5'
MvrI
PvuI
Isoschizomer
5'… CGAT▼CG …3'
3'… GC▲TAGC …5'
Pps I
5'… GAGTC(N)4▼ …3'
3'… CTCAG(N)5▲ …5'
PleI
Isoschizomer
5'… GAGTC(N)4▼ …3'
3'… CTCAG(N)5▲ …5'
MlyI
Neoschizomer
5'… GAGTC(N)5▼ …3'
3'… CTCAG(N)5▲ …5'
SchI
Neoschizomer
5'… GAGTC(N)5▼ …3'
3'… CTCAG(N)5▲ …5'
Pse31 I
5'… GGTCTC(N)1▼ …3'
3'… CCAGAG(N)5▲ …5'
Eco31I
Isoschizomer
5'… GGTCTC(N)1▼ …3'
3'… CCAGAG(N)5▲ …5'
PspC I
5'… CAC▼GTG …3'
3'… GTG▲CAC …5'
AcvI
BbrPI
Eco72I
PmaCI
PmlI
Isoschizomer
5'… CAC▼GTG …3'
3'… GTG▲CAC …5'
PspE I
5'… G▼GTNACC …3'
3'… CCANTG▲G …5'
BstEII
BstPI
Eco91I
EcoO65I
Isoschizomer
5'… G▼GTNACC …3'
3'… CCANTG▲G …5'
PspL I
5'… C▼GTACG …3'
3'… GCATG▲C …5'
BsiWI
Pfl23II
SplI
SunI
Isoschizomer
5'… C▼GTACG …3'
3'… GCATG▲C …5'
PspN4 I
5'… GGN▼NCC …3'
3'… CCN▲NGG …5'
BspLI
NlaIV
Isoschizomer
5'… GGN▼NCC …3'
3'… CCN▲NGG …5'
PspPP I
5'… RG▼GWCCY …3'
3'… YCCWG▲GR …5'
PpuMI
PpuXI
Psp5II
Isoschizomer
5'… RG▼GWCCY …3'
3'… YCCWG▲GR …5'
Pst I
5'… CTGCA▼G …3'
3'… G▲ACGTC …5'
BspMAI
Isoschizomer
5'… CTGCA▼G …3'
3'… G▲ACGTC …5'
PstN I
5'… CAGNNN▼CTG …3'
3'… GTC▲NNNGAC …5'
AlwNI
Isoschizomer
5'… CAGNNN▼CTG …3'
3'… GTC▲NNNGAC …5'
Rig I
5'… GGCCGG▼CC …3'
3'… CC▲GGCCGG …5'
FseI
Isoschizomer
5'… GGCCGG▼CC …3'
3'… CC▲GGCCGG …5'
Rsa I
5'… GT▼AC …3'
3'… CA▲TG …5'
AfaI
Isoschizomer
5'… GT▼AC …3'
3'… CA▲TG …5'
Csp6I
Neoschizomer
5'… G▼TAC …3'
3'… CAT▲G …5'
Rsr2 I
5'… CG▼GWCCG …3'
3'… GCCWG▲GC …5'
CpoI
CspI
RsrII
Isoschizomer
5'… CG▼GWCCG …3'
3'… GCCWG▲GC …5'
Sbf I
5'… CCTGCA▼GG …3'
3'… GG▲ACGTCC …5'
SdaI
Sse8387I
Isoschizomer
5'… CCTGCA▼GG …3'
3'… GG▲ACGTCC …5'
SfaN I
5'… GCATC(N)5▼ …3'
3'… CGTAG(N)9▲ …5'
LweI
Isoschizomer
5'… GCATC(N)5▼ …3'
3'… CGTAG(N)9▲ …5'
Sfr274 I
5'… C▼TCGAG …3'
3'… GAGCT▲C …5'
BssHI
PaeR7I
SlaI
StrI
TliI
XhoI
Isoschizomer
5'… C▼TCGAG …3'
3'… GAGCT▲C …5'
Sfr303 I
5'… CCGC▼GG …3'
3'… GG▲CGCC …5'
Cfr42I
KspI
SacII
SgrBI
SstII
Isoschizomer
5'… CCGC▼GG …3'
3'… GG▲CGCC …5'
Sma I
5'… CCC▼GGG …3'
3'… GGG▲CCC …5'
Cfr9I
XmaCI
Xma I
Neoschizomer
5'… C▼CCGGG …3'
3'… GGGCC▲C …5'
Smi I
5'… ATTT▼AAAT …3'
3'… TAAA▲TTTA …5'
SwaI
Isoschizomer
5'… ATTT▼AAAT …3'
3'… TAAA▲TTTA …5'
SmiM I
5'… CAYNN▼NNRTG …3'
3'… GTRNN▲NNYAC …5'
MslI
Isoschizomer
5'… CAYNN▼NNRTG …3'
3'… GTRNN▲NNYAC …5'
Sph I
5'… GCATG▼C …3'
3'… C▲GTACG …5'
BbuI
PaeI
SpaHI
Isoschizomer
5'… GCATG▼C …3'
3'… C▲GTACG …5'
Sse9 I
5'… ▼AATT …3'
3'… TTAA▲ …5'
TasI
Tsp509I
TspEI
Isoschizomer
5'… ▼AATT …3'
3'… TTAA▲ …5'
SspM I
5'… C▼TAG …3'
3'… GAT▲C …5'
MaeI
Isoschizomer
5'… C▼TAG …3'
3'… GAT▲C …5'
Tru9 I
5'… T▼TAA …3'
3'… AAT▲T …5'
MseI
Tru1I
Isoschizomer
5'… T▼TAA …3'
3'… AAT▲T …5'
TseF I
5'… ▼GTSAC …3'
3'… CASTG▲ …5'
Tsp45I
Isoschizomer
5'… ▼GTSAC …3'
3'… CASTG▲ …5'
Tth111 I
5'… GACN▼NNGTC …3'
3'… CTGNN▲NCAG …5'
AspI
PflFI
PsyI
TelI
Isoschizomer
5'… GACN▼NNGTC …3'
3'… CTGNN▲NCAG …5'
Vne I
5'… G▼TGCAC …3'
3'… CACGT▲G …5'
Alw44I
ApaLI
Isoschizomer
5'… G▼TGCAC …3'
3'… CACGT▲G …5'
Vsp I
5'… AT▼TAAT …3'
3'… TAAT▲TA …5'
AseI
PshBI
Isoschizomer
5'… AT▼TAAT …3'
3'… TAAT▲TA …5'
Zrm I
5'… AGT▼ACT …3'
3'… TCA▲TGA …5'
AssI
ScaI
Isoschizomer
5'… AGT▼ACT …3'
3'… TCA▲TGA …5'
Zsp2 I
5'… ATGCA▼T …3'
3'… T▲ACGTA …5'
EcoT22I
Mph1103I
NsiI
Isoschizomer
5'… ATGCA▼T …3'
3'… T▲ACGTA …5'
BfrBI
Neoschizomer
5'… ATGCA▼T …3'
3'… T▲ACGTA …5'
// Код для некролога