SibEnzyme restriction enzymes database

Список ферментов

Pvu II

Enzyme name Pvu II
Prototype PvuII
SKU SE-E111
Turbo version Available
High-concentration version Not available
Recognition site
5'… CAG▼CTG …3'
3'… GTC▲GAC …5'
Source An E.coli strain that carries the cloned Pvu II gene from Proteus vulgaris
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
25100252525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme approximately 70% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Repin, V.E., Degtyarev, S.Kh., Unpublished observations. V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

Поиск по названию

Pvu II

Enzyme name Pvu II
Prototype PvuII
SKU SE-E111
Turbo version Available
High-concentration version Not available
Recognition site
5'… CAG▼CTG …3'
3'… GTC▲GAC …5'
Source An E.coli strain that carries the cloned Pvu II gene from Proteus vulgaris
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
25100252525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme approximately 70% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Repin, V.E., Degtyarev, S.Kh., Unpublished observations. V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006