SibEnzyme restriction enzymes database

Список ферментов

RsaN I

Enzyme name RsaN I
Prototype RsaI
SKU SE-E555
Turbo version Not available
High-concentration version Not available
Recognition site
5'… G▼TAC …3'
3'… CAT▲G …5'
Source Rhodopseudomonas sphaeroides N
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
10075505075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes

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RsaN I

Enzyme name RsaN I
Prototype RsaI
SKU SE-E555
Turbo version Not available
High-concentration version Not available
Recognition site
5'… G▼TAC …3'
3'… CAT▲G …5'
Source Rhodopseudomonas sphaeroides N
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
10075505075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes