SibEnzyme restriction enzymes database

Список ферментов

Sal I

Enzyme name Sal I
Prototype SalI
SKU SE-E115
Turbo version Available
High-concentration version Not available
Recognition site
5'… G▼TCGAC …3'
3'… CAGCT▲G …5'
Source An E.coli strain that carries the cloned Sal I gene from Streptomyces albus
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
0101002505
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA pUC19 fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O
Notes High enzyme concentration may result in star activity.
References
  1. Arrand, J.R., Myers, P.A., Roberts, R.J., J. Mol. Biol. 118: 113-122 (1978).

Поиск по названию

Sal I

Enzyme name Sal I
Prototype SalI
SKU SE-E115
Turbo version Available
High-concentration version Not available
Recognition site
5'… G▼TCGAC …3'
3'… CAGCT▲G …5'
Source An E.coli strain that carries the cloned Sal I gene from Streptomyces albus
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
0101002505
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA pUC19 fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O
Notes High enzyme concentration may result in star activity.
References
  1. Arrand, J.R., Myers, P.A., Roberts, R.J., J. Mol. Biol. 118: 113-122 (1978).