SibEnzyme restriction enzymes database

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BamH I

Enzyme name BamH I
Prototype BamHI
SKU SE-E021
Turbo version Available
High-concentration version Available
Recognition site
5'… G▼GATCC …3'
3'… CCTAG▲G …5'
Source An E.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H
Optimal buffer SE-buffer G + BSA
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25100757525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligation After 50-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by Dam methylation (G m ATC) G GATC C
Supplied with enzyme 10 X SE-buffer G, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Wilson, G.A. and Young, F.E. J. Mol. Biol. 97: 123-125 (1975).Roberts, R.J., Wilson, G.A., Young, F.E. Nature 265: 82-84 (1977)

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BamH I

Enzyme name BamH I
Prototype BamHI
SKU SE-E021
Turbo version Available
High-concentration version Available
Recognition site
5'… G▼GATCC …3'
3'… CCTAG▲G …5'
Source An E.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H
Optimal buffer SE-buffer G + BSA
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25100757525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol. Store at -20°C.
Ligation After 50-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by Dam methylation (G m ATC) G GATC C
Supplied with enzyme 10 X SE-buffer G, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Wilson, G.A. and Young, F.E. J. Mol. Biol. 97: 123-125 (1975).Roberts, R.J., Wilson, G.A., Young, F.E. Nature 265: 82-84 (1977)