SibEnzyme restriction enzymes database

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Bgl I

Enzyme name Bgl I
Prototype BglI
SKU SE-E025
Turbo version Available
High-concentration version Not available
Recognition site
5'… GCCNNNN▼NGGC …3'
3'… CGGN▲NNNNCCG …5'
Source Bacillus globigii
Optimal buffer SE-buffer 2W
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
505007525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by dcm methylation (C m CWGG) G CCWGG NNGGC
Supplied with enzyme 10 X SE-buffer 2W
Notes High enzyme concentration may result in star activity
References
  1. Duncan, C.H., Wilson, G.A. and Young, F.E. J. Bacteriol. 134: 338 (1978).

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Bgl I

Enzyme name Bgl I
Prototype BglI
SKU SE-E025
Turbo version Available
High-concentration version Not available
Recognition site
5'… GCCNNNN▼NGGC …3'
3'… CGGN▲NNNNCCG …5'
Source Bacillus globigii
Optimal buffer SE-buffer 2W
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
505007525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by dcm methylation (C m CWGG) G CCWGG NNGGC
Supplied with enzyme 10 X SE-buffer 2W
Notes High enzyme concentration may result in star activity
References
  1. Duncan, C.H., Wilson, G.A. and Young, F.E. J. Bacteriol. 134: 338 (1978).