SibEnzyme restriction enzymes database

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BstSL I

Enzyme name BstSL I
Prototype BseSI
SKU SE-E561
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GKGCm▼C …3'
3'… C▲mCGKG …5'
Source Bacillus stearothermophilus S
Optimal buffer SE-buffer G
Optimal temperature 55 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50100507575100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these, 95% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity Not blocked by overlapping dcm-methylation (C m CWGG) GKGC CC WGG Blocked by GKG(5mC)MC methylation
Supplied with enzyme 10 X SE-buffer G, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.

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BstSL I

Enzyme name BstSL I
Prototype BseSI
SKU SE-E561
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GKGCm▼C …3'
3'… C▲mCGKG …5'
Source Bacillus stearothermophilus S
Optimal buffer SE-buffer G
Optimal temperature 55 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50100507575100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these, 95% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity Not blocked by overlapping dcm-methylation (C m CWGG) GKGC CC WGG Blocked by GKG(5mC)MC methylation
Supplied with enzyme 10 X SE-buffer G, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.