SibEnzyme restriction enzymes database

Список ферментов

Fal I

Enzyme name Fal I
Prototype FalI
SKU SE-E153
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼(N)8AAGNNNNNCTT(N)13▼ …3'
3'… ▲(N)13TTCNNNNNGAA(N)8▲ …5'
Source Flavobacterium aquatile Ob10
Optimal buffer SE-buffer W
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
025751005070
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 20% of the DNA fragments can be ligated and of these 80% can be recut. In the presence of 10%PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W, SAM
Notes High enzyme concentration results in star activity To obtain 100% activity, SAM should be added to a final concentration 0.01 mM
References
  1. Dedkov, V.S., Sinichkina, S.A., Abdurashitov, M.A., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).

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Fal I

Enzyme name Fal I
Prototype FalI
SKU SE-E153
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼(N)8AAGNNNNNCTT(N)13▼ …3'
3'… ▲(N)13TTCNNNNNGAA(N)8▲ …5'
Source Flavobacterium aquatile Ob10
Optimal buffer SE-buffer W
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
025751005070
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 20% of the DNA fragments can be ligated and of these 80% can be recut. In the presence of 10%PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W, SAM
Notes High enzyme concentration results in star activity To obtain 100% activity, SAM should be added to a final concentration 0.01 mM
References
  1. Dedkov, V.S., Sinichkina, S.A., Abdurashitov, M.A., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).