SibEnzyme restriction enzymes database

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Mbo II

Enzyme name Mbo II
Prototype MboII
SKU SE-E471
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GAAGA(N)8▼ …3'
3'… CTTCT(N)7▲ …5'
Source An E.coli strain, that carries the cloned gene Mbo II from Moraxella bovis
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7575255010050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dam-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme approximately 60% of the DNA fragments can be ligated and recut. In presence of 10%PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlappin dam-methylation(G m ATC) GAA GA TC.
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Brown N.L., Hutchison C.A. III, Smith M. J. Mol. Biol. 140: 143-148 (1980).

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Mbo II

Enzyme name Mbo II
Prototype MboII
SKU SE-E471
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GAAGA(N)8▼ …3'
3'… CTTCT(N)7▲ …5'
Source An E.coli strain, that carries the cloned gene Mbo II from Moraxella bovis
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7575255010050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dam-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme approximately 60% of the DNA fragments can be ligated and recut. In presence of 10%PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlappin dam-methylation(G m ATC) GAA GA TC.
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Brown N.L., Hutchison C.A. III, Smith M. J. Mol. Biol. 140: 143-148 (1980).