SibEnzyme restriction enzymes database

Список ферментов

PspX I

Enzyme name PspX I
Prototype PspXI
SKU SE-E477
Turbo version Available
High-concentration version Available
Recognition site
5'… VC▼TCGAGB …3'
3'… BGAGCT▲CV …5'
Source An E.coli strain that carries the cloned PspX I gene from Pseudomonas species A1-1
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
5050257510025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2 -mercaptoethanol, 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Gonchar D.A., Abdurashitov M.A., Belichenko O.A., Dedkov V.S., Mezentseva N.V., Tomilova J.E., Degtyarev S.Kh. Bulletin of biotechnology and physico-chemical biology named by Yu.A.Ovchinnikov, No. 1, pp18-23 (2005)

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PspX I

Enzyme name PspX I
Prototype PspXI
SKU SE-E477
Turbo version Available
High-concentration version Available
Recognition site
5'… VC▼TCGAGB …3'
3'… BGAGCT▲CV …5'
Source An E.coli strain that carries the cloned PspX I gene from Pseudomonas species A1-1
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
5050257510025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2 -mercaptoethanol, 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Gonchar D.A., Abdurashitov M.A., Belichenko O.A., Dedkov V.S., Mezentseva N.V., Tomilova J.E., Degtyarev S.Kh. Bulletin of biotechnology and physico-chemical biology named by Yu.A.Ovchinnikov, No. 1, pp18-23 (2005)