SibEnzyme restriction enzymes database

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Ssp I

Enzyme name Ssp I
Prototype SspI
SKU SE-E041
Turbo version Available
High-concentration version Not available
Recognition site
5'… AAT▼ATT …3'
3'… TTA▲TAA …5'
Source An E.coli strain, that carries the cloned gene SspI from Sphaerotilus species
Optimal buffer SE-buffer K
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7550255075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by methylation A m ATATT.
Supplied with enzyme 10 X SE-buffer K, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Schildkraut, I., Grandoni, R. unpublished observations. Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007

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Ssp I

Enzyme name Ssp I
Prototype SspI
SKU SE-E041
Turbo version Available
High-concentration version Not available
Recognition site
5'… AAT▼ATT …3'
3'… TTA▲TAA …5'
Source An E.coli strain, that carries the cloned gene SspI from Sphaerotilus species
Optimal buffer SE-buffer K
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7550255075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by methylation A m ATATT.
Supplied with enzyme 10 X SE-buffer K, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Schildkraut, I., Grandoni, R. unpublished observations. Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007