SibEnzyme restriction enzymes database

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Pec I

Enzyme name Pec I
Prototype PsiI
SKU SE-E601
Turbo version Available
High-concentration version Not available
Recognition site
5'… TTA▼TAA …3'
3'… AAT▲ATT …5'
Source Paracoccus species 12
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50751025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and of these 95% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25. PsiI
References
  1. Gonchar D.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Abdurashitov M.A., Belichenko O.A., Degtyarev S.Kh. (2024) The restriction endonuclease PecI from the bacterial strain Paracoccus species 12 recognizes the DNA sequence 5′-TTA^TAA-3′ and is an isoschizomer of PsiI. DNA-Processing Enzymes, 2024(1).

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Pec I

Enzyme name Pec I
Prototype PsiI
SKU SE-E601
Turbo version Available
High-concentration version Not available
Recognition site
5'… TTA▼TAA …3'
3'… AAT▲ATT …5'
Source Paracoccus species 12
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50751025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and of these 95% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25. PsiI
References
  1. Gonchar D.A., Chernukhin V.A., Dedkov V.S., Mikhnenkova N.A., Abdurashitov M.A., Belichenko O.A., Degtyarev S.Kh. (2024) The restriction endonuclease PecI from the bacterial strain Paracoccus species 12 recognizes the DNA sequence 5′-TTA^TAA-3′ and is an isoschizomer of PsiI. DNA-Processing Enzymes, 2024(1).