SibEnzyme restriction enzymes database

Список ферментов

Psi I

Enzyme name Psi I
Prototype PsiI
SKU SE-E279
Turbo version Available
High-concentration version Not available
Recognition site
5'… TTA▼TAA …3'
3'… AAT▲ATT …5'
Source Pseudomonas species-SE-G49
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1002510257540
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and of these 95% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.
References
  1. Abdurashitov, M.A., Belichenko, O.A., Lebedeva, N.A., Degtyarev, S.Kh. Biokhimiia 64: 574-576 (1999).

Поиск по названию

Psi I

Enzyme name Psi I
Prototype PsiI
SKU SE-E279
Turbo version Available
High-concentration version Not available
Recognition site
5'… TTA▼TAA …3'
3'… AAT▲ATT …5'
Source Pseudomonas species-SE-G49
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
1002510257540
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and of these 95% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.
References
  1. Abdurashitov, M.A., Belichenko, O.A., Lebedeva, N.A., Degtyarev, S.Kh. Biokhimiia 64: 574-576 (1999).